HCSGD entry for RUNX2

1. General information

Official gene symbolRUNX2
Entrez ID860
Gene full namerunt-related transcription factor 2
Other gene symbolsAML3 CBFA1 CCD CCD1 CLCD OSF-2 OSF2 PEA2aA PEBP2A1 PEBP2A2 PEBP2aA PEBP2aA1
Links to Entrez GeneLinks to Entrez Gene

2. Neighbors in the network

color bar

3. Gene ontology annotation

GO ID

GO term

Evidence

Category

GO:0000790Nuclear chromatinISScellular_component
GO:0000978RNA polymerase II core promoter proximal region sequence-specific DNA bindingIEAmolecular_function
GO:0001077RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcriptionIEAmolecular_function
GO:0001503OssificationTASbiological_process
GO:0001649Osteoblast differentiationIEP TASbiological_process
GO:0001958Endochondral ossificationIEAbiological_process
GO:0002051Osteoblast fate commitmentIEAbiological_process
GO:0002063Chondrocyte developmentIEAbiological_process
GO:0002076Osteoblast developmentIEAbiological_process
GO:0003677DNA bindingIEAmolecular_function
GO:0003682Chromatin bindingIEAmolecular_function
GO:0003700Sequence-specific DNA binding transcription factor activityIEA NASmolecular_function
GO:0005515Protein bindingIPImolecular_function
GO:0005524ATP bindingIEAmolecular_function
GO:0005634NucleusIDA IEAcellular_component
GO:0005667Transcription factor complexIEAcellular_component
GO:0005730NucleolusIDAcellular_component
GO:0005737CytoplasmIEAcellular_component
GO:0006351Transcription, DNA-templatedIEAbiological_process
GO:0006367Transcription initiation from RNA polymerase II promoterTASbiological_process
GO:0008284Positive regulation of cell proliferationIEAbiological_process
GO:0010467Gene expressionTASbiological_process
GO:0019904Protein domain specific bindingIEAmolecular_function
GO:0030217T cell differentiationIEAbiological_process
GO:0030509BMP signaling pathwayISSbiological_process
GO:0032332Positive regulation of chondrocyte differentiationIEAbiological_process
GO:0035115Embryonic forelimb morphogenesisIEAbiological_process
GO:0040036Regulation of fibroblast growth factor receptor signaling pathwayIEAbiological_process
GO:0042475Odontogenesis of dentin-containing toothIEAbiological_process
GO:0042487Regulation of odontogenesis of dentin-containing toothIEAbiological_process
GO:0043425BHLH transcription factor bindingIEAmolecular_function
GO:0045669Positive regulation of osteoblast differentiationIEAbiological_process
GO:0045879Negative regulation of smoothened signaling pathwayIEAbiological_process
GO:0045892Negative regulation of transcription, DNA-templatedIDAbiological_process
GO:0045893Positive regulation of transcription, DNA-templatedIDAbiological_process
GO:0048469Cell maturationIEAbiological_process
GO:0048701Embryonic cranial skeleton morphogenesisIEAbiological_process
GO:0048863Stem cell differentiationIEAbiological_process
GO:0070491Repressing transcription factor bindingIEAmolecular_function
GO:0071773Cellular response to BMP stimulusISSbiological_process
GO:1901522Positive regulation of transcription from RNA polymerase II promoter involved in cellular response to chemical stimulusISSbiological_process
Entries Per Page
Displaying Page of

4. Expression levels in datasets

  • Meta-analysis result

p-value upp-value downFDR upFDR down
0.72965803730.16249659590.99999024730.7786406153

  • Individual experiment result
    ( "-" represent NA in the specific microarray platform )

Data sourceUp or downLog fold change
GSE11954Down-0.1106753196
GSE13712_SHEARDown-0.0118709569
GSE13712_STATICUp0.0117738076
GSE19018Down-0.1581816805
GSE19899_A1Up0.2281252622
GSE19899_A2Down-0.3260065227
PubMed_21979375_A1Up0.1900307949
PubMed_21979375_A2Down-0.1537739019
GSE35957Down-0.1499243173
GSE36640Down-0.3115378953
GSE54402Up0.3809699804
GSE9593Down-0.4792662912
GSE43922Down-0.2366107107
GSE24585Down-0.0082646626
GSE37065Down-0.1678193310
GSE28863_A1Up0.1353121024
GSE28863_A2Up0.4481141480
GSE28863_A3Up0.1592869617
GSE28863_A4Down-0.3389447778
GSE48662Down-0.1171350969

5. Regulation relationships with compounds/drugs/microRNAs

  • Compounds

Not regulated by compounds

  • Drugs

Not regulated by drugs

  • MicroRNAs

  • mirTarBase

MiRNA_name

mirBase ID

miRTarBase ID

Experiment

Support type

References (Pubmed ID)

hsa-miR-135b-5pMIMAT0000758MIRT003597qRT-PCRNon-Functional MTI (Weak)19795981
hsa-miR-155-5pMIMAT0000646MIRT004707Luciferase reporter assay//qRT-PCR//Western blotFunctional MTI20427544
hsa-miR-335-5pMIMAT0000765MIRT005888Luciferase reporter assay//Microarray//qRT-PCR//Western blotFunctional MTI21164520
hsa-miR-204-5pMIMAT0000265MIRT006895Luciferase reporter assay//qRT-PCR//Western blotFunctional MTI22871591
hsa-miR-433-3pMIMAT0001627MIRT007225Luciferase reporter assayFunctional MTI23353875
hsa-miR-338-3pMIMAT0000763MIRT007289Mass spectrometryFunctional MTI (Weak)23380982
hsa-miR-124-3pMIMAT0000422MIRT022898MicroarrayFunctional MTI (Weak)18668037
hsa-miR-30d-5pMIMAT0000245MIRT025969Reporter assay;Western blotFunctional MTI21767385
hsa-miR-30a-5pMIMAT0000087MIRT028618Reporter assay;Western blotFunctional MTI21767385
hsa-miR-505-5pMIMAT0004776MIRT037957CLASHFunctional MTI (Weak)23622248
hsa-miR-484MIMAT0002174MIRT041692CLASHFunctional MTI (Weak)23622248
hsa-miR-30b-5pMIMAT0000420MIRT046115CLASHFunctional MTI (Weak)23622248
Entries Per Page
Displaying Page of
  • mirRecord
No target information from mirRecord

6. Text-mining results about the gene

Gene occurances in abstracts of cellular senescence-associated articles: 15 abstracts the gene occurs.


PubMed ID of the article

Sentenece the gene occurs

27165403The onset of senescence inhibited both the induction of osteoblast markers RUNX2 and osteopontin and the biomineralization of DFCs after stimulation of the osteogenic differentiation
25922305Based on the production of ECM proteins, such as fibronectin, integrin beta1, and collagen type I; alkaline phosphatase (ALP) activity; and the expression of osteogenic genes, such as ALP, Runt-related transcription factor 2, and osteocalcin, cell sheets formed by PDLSCs derived from older donors demonstrated a less potent osteogenic capacity compared to those formed by PDLSCs from younger donors
25342130The increase in calcification was accompanied by up-regulation of Cbfa1 (osteogenic transcription factor) and down-regulation of SM22alpha (VSMC lineage marker)
23934584Runx2, a down-regulation which was found to be p53-dependent
23804221The CD73(+)CD39(+) cell subset displayed higher expression levels of Sox9 and Runx2 and a significantly greater chondro-osteogenic potency than the CD73(+)CD39(-) cell subset
23611899RESULTS: Both IL-1beta and TNFalpha-induced strong expression of multiple MMPs and hypertrophic markers Runx2 and type X collagen
22738657Oxidative stress-related signals and some microRNAs affect the differentiation potential shift of MSC by directly targeting key regulatory factors such as Runx-2 or PPAR-gamma, and energy metabolism pathway is involved as well
22621437Telomere-mediated defects in osteoblast differentiation are associated with increased p53/p21 expression and concomitant reduction in RUNX2
22133824In addition, the activation of Runx2, a potent osteogenic transcriptional factor, in SMC is regulated by SIRT1-p21 axis
21912699This led to maintenance osteoblastic cell development and differentiation and delay of cellular entrance into senescence through regulation of the Runx2 gene
21694780We found that MSCs underwent aging and spontaneous osteogenic differentiation upon regular culture expansion, with progressive downregulation of TERT and upregulation of osteogenic genes such as Runx2 and ALP
20647039Senescent VSMCs have been shown to overexpress genes and proteins (including RUNX-2, alkaline phosphatase (ALP), type I collagen and BMP-2) associated with osteoblasts, leading to partial osteoblastic transdifferentiation
19749165Of note, runt-related transcription factor-2 (RUNX-2), a core transcriptional factor that initiates the osteoblastic differentiation, was also upregulated in the senescent VSMCs
19749165Knockdown of RUNX-2 significantly reduced the ALP expression and calcification in the senescent VSMCs, suggesting that RUNX-2 is involved in the senescence-mediated osteoblastic transition
19749165Furthermore, immunohistochemistry of aorta from the klotho(-/-) aging mouse model demonstrated in vivo emergence of osteoblast-like cells expressing RUNX-2 exclusively in the calcified media
17352650We observed an inverse expression pattern between the osteogenic master regulatory gene, CBFA1, and the stem cell-associated gene, hTERT
17352650We showed that Cbfa1 acts as a partial repressor of TERT, which may facilitate cellular differentiation
17352650Ex vivo expansion of BMSSCs correlates to an increase in osteogenic lineage associated markers such as alkaline phosphatase, bone sialoprotein, and osteocalcin that are regulated by the master regulatory transcription factor, Cbfa1 (Runx2)
17352650This study examined whether Cbfa1 was capable of regulating the promoter of the early stem cell-associated gene, telomerase reverse transcriptase (TERT)
17352650CBFA1 and TERT gene expression was assessed by real-time PCR
17352650The functional capacity of Cbfa1 to bind to the hTERT promoter was performed using a modified electrophoretic mobility shift assay (EMSA)
17352650Chromatin immunoprecipitation (ChIP) analysis was used to examine Cbfa1 binding to the hTERT promoter in vivo
17352650Functional analysis of CBFA-1 wildtype and mutant DNA binding sites on TERT promoter fragments was assessed using the promoterless green fluorescence protein (GFP) reporter vector, pEGFP-1, after transfection into HOS cells
17352650RESULTS: This study showed an inverse expression pattern between the osteogenic master regulatory gene, CBFA1, and the stem cell-associated gene, hTERT
17352650The data showed that BMSSCs undergo osteogenic commitment after the loss of hTERT expression, with concomitant elevated levels of CBFA1 transcripts
17352650In addition, two unique Cbfa1 DNA binding sites were identified on the hTERT proximal promoter by EMSA supershift assay
17352650Mutated forms of the putative Cbfa1 binding sites, created by site-directed mutagenesis, were able to abolish this interaction
17352650ChIP analysis showed that Cbfa1 interacted directly with the hTERT promoter in vivo
17352650Functional studies using GFP reporter constructs, driven by 2- and 3-kbp hTERT proximal promoter fragments, showed significantly lower levels of transcriptional activity compared with corresponding constructs with mutated Cbfa1 binding site Oligo 2
17352650CONCLUSIONS: These studies suggest that Cbfa1 may act as a repressor of early stem cell markers such as hTERT as one possible mechanism for facilitating cellular differentiation
10811146CBFA1 and topoisomerase I mRNA levels decline during cellular aging of human trabecular osteoblasts
10811146In order to understand the reasons for age-related impairment of the function of bone forming osteoblasts, we have examined the steady-state mRNA levels of the transcription factor CBFA1 and topoisomerase I during cellular aging of normal human trabecular osteoblasts, by the use of semiquantitative reverse transcriptase-polymerase chain reaction (RT-PCR)
10811146There is a progressive and significant reduction of the CBFA1 steady-state mRNA level down to 50% during cellular aging of human osteoblasts
10811146In comparison to the normal cells, human osteosarcoma cell lines SaOS-2 and KHOS/NP, and the SV40-transformed human lung fibroblast cell line MRC5V2 have 20 to 40% higher levels of CBFA1 mRNA
10811146Similar levels of CBFA1 mRNA are detectable in normal human skin fibroblasts, and these cells also exhibit an age-related decline to the same extent
Entries Per Page
Displaying Page of